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BPA exposure induces mitochondrial dysfunction, mitigated by NaOx or AC45594 HESC were treated with CTL or 50 nM BPA for 3 days, followed by 3-day treatment with vehicle, NaOx (20 mM), or AC45594 (5 μM). (A) Intracellular ATP. (B) Mitochondrial DNA copy number. (C) Mitochondrial membrane potential (ΔΨm). (D) Representative TMRE images. (E) Caspase-3 activity. (F) Apoptosis rate. n means number of independent repeats, n = 5. ∗, p < 0.05 vs. CTL(3d)+CTL(3d)/VEH; ¶, p < 0.05 vs. BPA(3d) + CTL(3d)/VEH. Data are presented as mean ± SD.

Journal: iScience

Article Title: Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibility

doi: 10.1016/j.isci.2026.115608

Figure Lengend Snippet: BPA exposure induces mitochondrial dysfunction, mitigated by NaOx or AC45594 HESC were treated with CTL or 50 nM BPA for 3 days, followed by 3-day treatment with vehicle, NaOx (20 mM), or AC45594 (5 μM). (A) Intracellular ATP. (B) Mitochondrial DNA copy number. (C) Mitochondrial membrane potential (ΔΨm). (D) Representative TMRE images. (E) Caspase-3 activity. (F) Apoptosis rate. n means number of independent repeats, n = 5. ∗, p < 0.05 vs. CTL(3d)+CTL(3d)/VEH; ¶, p < 0.05 vs. BPA(3d) + CTL(3d)/VEH. Data are presented as mean ± SD.

Article Snippet: ApoAlert Caspase-3 Activity Kit , Clontech , Cat# 630216.

Techniques: Membrane, Activity Assay

Effect of curcumin, spanlastics-free, curcumin nanocrystals, and curcumin spanlastics on Caspase 3 activity using Caspase 3 Activity Assay Kit (Colorimetric) on the IC50 concentration detected by MTT viability test (IC50 = 240.85, 67.7, 47.83, and 37.18 µg/mL µg/mL, respectively, incubation for 48 h). Data were expressed as mean ± SD ( n = 3). a means significant versus the control group and b means significant versus the curcumin group. Control: cancer cell without any treatments. Each group differed significantly from the others at p ≤ 0.05

Journal: BMC Biotechnology

Article Title: An assessment of curcumin, curcumin spanlastics nanoparticles, and curcumin nanocrystals as possible drug delivery systems with antimicrobial, antioxidant, and antitumor activities

doi: 10.1186/s12896-026-01153-x

Figure Lengend Snippet: Effect of curcumin, spanlastics-free, curcumin nanocrystals, and curcumin spanlastics on Caspase 3 activity using Caspase 3 Activity Assay Kit (Colorimetric) on the IC50 concentration detected by MTT viability test (IC50 = 240.85, 67.7, 47.83, and 37.18 µg/mL µg/mL, respectively, incubation for 48 h). Data were expressed as mean ± SD ( n = 3). a means significant versus the control group and b means significant versus the curcumin group. Control: cancer cell without any treatments. Each group differed significantly from the others at p ≤ 0.05

Article Snippet: Caspase 3 Activity was estimated through Caspase 3 Activity Assay Kit (Colorimetric), NBP2-54838-100 Assays, Novus Biologicals LLC, a Bio-Techne Brand, according to manufacturer protocol through HiPo MPP-96 Microplate Photometer and 3D-IW8 Inteliwasher (Biosan).

Techniques: Activity Assay, Caspase-3 Activity Assay, Concentration Assay, Incubation, Control